Spectral-lifetime Unmixing Algorithms for Biological Fluorescence Imaging
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منابع مشابه
Spectral Separation of Quantum Dots within Tissue Equivalent Phantom Using Linear Unmixing Methods in Multispectral Fluorescence Reflectance Imaging
Introduction Non-invasive Fluorescent Reflectance Imaging (FRI) is used for accessing physiological and molecular processes in biological media. The aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using SVD, Jacobi SVD, and NMF methods in the FRI mode. Materials and Methods In this article, a tissue-like phantom and an optical...
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The ability to quantify the fluorescence signals from multiply labeled biological samples is highly desirable in the life sciences but often difficult, because of spectral overlap between fluorescent species and the presence of autofluorescence. Several so called unmixing algorithms have been developed to address this problem. Here, we present a novel algorithm that combines measurements of lif...
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Fluorescence lifetime imaging microscopy (FLIM) is a quantitative microscopy technique for imaging nanosecond decay times of fluorophores. In the case of frequency-domain FLIM, several methods have been described to resolve the relative abundance of two fluorescent species with different fluorescence decay times. Thus far, single-frequency FLIM methods generally have been limited to quantifying...
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Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows the creation of increasingly complex multicolored samples. A reliable separation of the different fluorescence labels is required for analysis and quantitation, but it is complicated by the significant overlap of the emission spectra. This problem ...
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BACKGROUND Nonnegative matrix factorization (NMF) has been used in blind fluorescence unmixing for multispectral in-vivo fluorescence imaging, which decomposes a mixed source data into a set of constituent fluorescence spectra and corresponding concentrations. However, most classical NMF algorithms have ill convergence problems and they always fail to unmix multiple fluorescent targets from bac...
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تاریخ انتشار 2009